Epidermal wound repair is regulated by the planar cell polarity signaling pathway. / Caddy, Jacinta; Wilanowski, Tomasz; Darido, Charbel; Dworkin, Sebastian; Ting, Stephen B.; Zhao, Quan; Rank, Gerhard; Auden, Alana; Srivastava, Seema; Papenfuss, Tony A.; Murdoch, Jennifer N.; Humbert, Patrick O.; Boulos, Nidal; Weber, Thomas; Zuo, Jian; Cunningham, John M.; Jane, Stephen M.

In: Developmental Cell, Vol. 19, No. 1, 20.07.2010, p. 138-147.

Research output: Contribution to journalArticle

Published

  • Jacinta Caddy
  • Tomasz Wilanowski
  • Charbel Darido
  • Sebastian Dworkin
  • Stephen B. Ting
  • Quan Zhao
  • Gerhard Rank
  • Alana Auden
  • Seema Srivastava
  • Tony A. Papenfuss
  • Jennifer N. Murdoch
  • Patrick O. Humbert
  • Nidal Boulos
  • Thomas Weber
  • Jian Zuo
  • John M. Cunningham
  • Stephen M. Jane

Abstract

The mammalian PCP pathway regulates diverse developmental processes requiring coordinated cellular movement, including neural tube closure and cochlear stereociliary orientation. Here, we show that epidermal wound repair is regulated by PCP signaling. Mice carrying mutant alleles of PCP genes Vangl2, Celsr1, PTK7, and Scrb1, and the transcription factor Grhl3, interact genetically, exhibiting failed wound healing, neural tube defects, and disordered cochlear polarity. Using phylogenetic analysis, ChIP, and gene expression in Grhl3(-)(/-) mice, we identified RhoGEF19, a homolog of a RhoA activator involved in PCP signaling in Xenopus, as a direct target of GRHL3. Knockdown of Grhl3 or RhoGEF19 in keratinocytes induced defects in actin polymerization, cellular polarity, and wound healing, and re-expression of RhoGEF19 rescued these defects in Grhl3-kd cells. These results define a role for Grhl3 in PCP signaling and broadly implicate this pathway in epidermal repair.
Original languageEnglish
Pages138-147
Number of pages10
JournalDevelopmental Cell
Journal publication date20 Jul 2010
Journal number1
Volume19
DOIs
StatePublished

ID: 1143279