Production of non viral DNA vectors. / Schleef, Martin; Blaesen, Markus; Schmeer, Marco; Baier, Ruth; Marie, Corinne; Dickson, George; Scherman, Daniel.

In: Current Gene Therapy, Vol. 10, No. 6, 2010, p. 487-507.

Research output: Contribution to journalArticle

Published

  • Martin Schleef
  • Markus Blaesen
  • Marco Schmeer
  • Ruth Baier
  • Corinne Marie
  • George Dickson
  • Daniel Scherman

Abstract

After some decades of research, development and first clinical approaches to use DNA vectors in gene therapy, cell therapy and DNA vaccination, the requirements for the pharmaceutical manufacturing of gene vectors has improved significantly step by step. Even the expression level and specificity of non viral DNA vectors were significantly modified and followed the success of viral vectors. The strict separation of "viral" and "non viral" gene transfer are historic borders between scientist and we will show that both fields together are able to allow the next step towards successful prevention and therapy. Here we summarize the features of producing and modifying these non-viral gene vectors to ensure the required quality to modify cells and to treat human and animals.
Original languageEnglish
Pages487-507
Number of pages21
JournalCurrent Gene Therapy
Journal publication date2010
Journal number6
Volume10
StatePublished

ID: 1197524